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GENTREE Final Conference : Anna-Maria FARSAKOGLOU - Aristotle University of Thessaloniki · Greece GENTREE Final Conference 'Genetics to the rescue - managing forests sustainably in a changing environment' 27-31 January 2020, Avignon, France http://www.gentree-h2020.eu/ Anna-Maria FARSAKOGLOU - Aristotle University of Thessaloniki · Greece : Genome-wide genetic diversity and differentiation of 12 major European tree species elucidates conservation needs The prospect of genetic diversity loss under a changing climate calls for improved conservation strategies. Recent technological advances enabled us to estimate with a high analytical power, genetic diversity and differentiation parameters at the genome-wide level and answer crucial biological questions related to conservation and to the fate of populations under strong environmental change. Herein, we present key genetic diversity and genetic differentiation parameters for 12 ecologically significant and commercially important European tree species, as the baseline of a concrete plan of action for the conservation of the European forest genetic resources. Material/Methods: For each species 10 to 25 populations (19 to 25 trees per population) were used for Allegro genotyping and exome capture sequencing, respectively, in order to produce around several thousands SNPs for each species. A bioinformatics pipeline was constructed, using the R and UNIX environments, in order to automatically analyze the data and estimate genetic parameters. Results: To assess genetic variation from a conservation perspective, we estimated for all populations of each species, expected (He) and observed (Ho) heterozygosity, the inbreeding coefficient (FIS), and the FST(i) as the genetic differentiation of a single population compared to all others within a species. Moreover, we have also calculated the Effective Population Size (Ne), and the folded Site Frequency Spectrum (SFS) with its respective concavity, to infer a possible signal of underlying evolutionary forces. Conclusion: A set, and overview of conservation genetics parameters is provided for a network of natural populations across the species range for some of the most important forest trees in Europe. ?hese parameters will be useful to highlight population properties, such as high genetic diversity, or severe genetic bottlenecks and conceive strategies ranging from in situ gene conservation to genetic rescue. Therefore, these results can serve as a powerful tool for novel approaches in conservation and management.

"Domaine(s)" et indice(s) Dewey

• SCIENCES nature - Botanique Arbres, Arbustes, Plantes grimpantes (580.16)

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  • GENTREE Scientific Conference - Genetics to the rescue : managing forests sustainably in a changing world